Part:BBa_K1797001

Red Light Sensing System

Fig.1 Principles of red light sensing system (source: Levskaya A, Chevalier A A, Tabor J J, et al. Synthetic biology: engineering Escherichia coli to see light[J]. Nature, 2005, 438(7067): 441-442.)

This part is mainly composed of Cph8(BBa_K592018), ho1(BBa_I15008) and PcyA(BBa_I15009), which form the red light sensing system in E.coli. Cph8 is a synthetic red light sensor, consisting of Cph1, a natural red light sensor, and EnvZ, a histidine kinase. The most important part of the light sensor is PCB(phycocyanobilin), without which the red light sensor cannot work. However, PCB is not generated by E.coli naturally. So we need to introduce ho1 and PcyA into E.coli. Actually we've also submitted the PCB generation system(BBa_K1797008). These two enzymes can change heme into PCB. Here we combine the three main parts- Cph8, ho1 and PcyA- together to facilitate parts users.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 3871
  Illegal XhoI site found at 425
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 3424
  
• 1000
  COMPATIBLE WITH RFC[1000]